In vitro BBB Technology

Every year millions of dollars are spent by pharmaceutical companies to develop and test drugs using in vitro models of the Blood-Brain Barrier (BBB). Many of these cases end up not working in vivo. We formed Flocel Inc, to manufacture a practical in vitro model that more accurately reflects the BBB characteristics. Flocel's dynamic in vitro blood-brain barrier (DIV-BBB) technology helps researchers in the United States and Europe conduct in vitro studies. Every year millions of dollars are spent by pharmaceutical companies to develop and test drugs using in vitro models of the Blood-Brain Barrier (BBB). Many of these cases end up not working in vivo. We formed Flocel Inc, to manufacture a practical in vitro model that more accurately reflects the BBB characteristics. Flocel's dynamic in vitro blood-brain barrier (DIV-BBB) technology helps researchers in the United States and Europe conduct in vitro studies.

The blood-brain barrier (BBB) explains why certain substances circulating in the blood are able to get into the brain and others do not. As determined by electron microscopy, the BBB is formed by brain capillaries that look like simple tubes of endothelial cells linked by tight junctions. The Flocel in vitro BBB is a tool that allows to reproduce in a flow-based 3D cell culture apparatus the essential properties of the human BBB, including the expression of metabolic and extrusion mechanisms responsible for multiple drug resistance to antiepileptic drugs, anti-tumor agents, etc.

The Flocel apparatus consists of hollow fibers (the artificial blood vessels) that are surrounded by an extracapillary space (the brain side). In addition to the cerebrovascular system, this apparatus can also be used to mimic the bone marrow, the placental barrier, or the CSF-blood barrier. In the case of modeling of bone marrow, the abluminal space is an ideal environment for the growth and differentiation of stem cells.

The Flocel DIV-BBB package contains: in vitro cell culture modules, a computer-controlled perfusion system, and a trans-endothelial electrical resistance (TEER) measurement module.

Download the Flocel User Manual

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