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- In Vitro 3D Cell Culture: An Overview - 

Each year pharmaceutical companies spend millions of dollars to test drugs using in vitro models of the blood-brain barrier (BBB). Flocel’s family of Dynamic In Vitro Blood-Brain Barrier (DIV-BBB) products offer a superior approach to this method by utilizing human endothelial cells and formation of tight junctions within its dynamic system, resulting in a physiologically accurate reflection of BBB characteristics.

In Vitro 3D Cell Culture Technology

The DIV-BBB 2.0 enables more accurate pharmacokinetic and toxicological studies and reflects in vivo blood-brain barrier properties. Its replication of important endothelial cell-astrocyte interactions and ability to sustain up to four cell culture modules substantially reduces drug development cost and experimental time. In addition to the cerebrovascular system, the cell culture modules allow for pericyte, epithelial and tumor cell growth, as well as modeling of bone marrow, the placental barrier, the blood-CSF barrier, and much more!

   

  

- Our Product Lines - 

      

- All Flocel Products and Systems -

    

   

PLEASE NOTE: In order to have Flocel products gas sterilized, the customer MUST specify this prior to confirming their purchase. Flocel products are delivered unsterilized and -unless otherwise requested by the customer- must be prepared post-delivery. 

   

  

To receive a quote for the products listed below, check the boxes next to the items of interest, enter your information, and Submit.

DIV-BBB 2.0, Multiplex Module Assembly:
     - one TEER Measurement Unit (TRM1010).
     - one Fluid Perfusion Unit (QPS1010 or QPL1010).
     - four cell culture modules (CAR1010 or CAR1020).
     - other module combinations available upon request.
     - all associated glassware, connectors, and tubing.
     - lifetime software updates, distributed online.
     - 1 year warranty on all hardware components.
     
Applications:

     - in vitro culture and TEER measurement of up to 4 cell culture modules.
     - see Cell Culture menu for experimental capabilities of modules.

DIV-BBB 2.0, Piezo Micro-Pump Assembly:
     - one TEER Measurement Unit (TRS1010).
     - one Piezo Micro-Pump Unit (QPP1010).
     - one cell culture module (CAR1060).
     - other modules available upon request.
     - all associated glassware, connectors, and tubing.
     - lifetime software updates, distributed online.
     - 1 year warranty on all hardware components.
     
Applications:

     - in vitro culture and TEER measurement for one cell culture module.
     - see Cell Culture menu for experimental capabilities of modules.

TRS1010:
     - transendothelial electrical resistance measurement unit.
     - services one in vitro cell culture module at a time.
     - frequency sampling 0.1 -1,000 Hz (0.1 Hz resolution).
     - excitation signal 5 - 60 mV p-p (0.1 mV resolution).
     - automated frequency sweeps and time sweeps.
     - automated data logging to an Excel or .csv file. 
     - lifetime software updates, distributed online.
     
Applications:

     - in vitro TEER measurement of one cell culture module.

TRM1010:
     - transendothelial electrical resistance measurement unit.
     - services up to four in vitro cell culture modules at a time.
     - frequency sampling 0.1 -1,000 Hz (0.1 Hz resolution).
     - excitation signal 5 - 60 mV p-p (0.1 mV resolution).
     - automated frequency sweeps and time sweeps.
     - automated data logging to an Excel or .csv file. 
     - lifetime software updates, distributed online.
     
Applications:

     - in vitro TEER measurement of up to four cell culture modules.

TRT1010:
     - transendothelial electrical resistance measurement unit.
     - services one Endohm cell culture cup chamber at a time.
     - frequency sampling 0.1 -1,000 Hz (0.1 Hz resolution).
     - excitation signal 5 - 60 mV p-p (0.1 mV resolution).
     - automated frequency sweeps and time sweeps.
     - automated data logging to an Excel or .csv file. 
     - lifetime software updates, distributed online.
     
Applications:

     - TEER measurement for one Endohm-6, Endohm-12 or Endohm-24SNAP chamber.

TRT1020:
     - transendothelial electrical resistance measurement unit.
     - services up to four Endohm cell culture cup chambers at a time.
     - frequency sampling 0.1 -1,000 Hz (0.1 Hz resolution).
     - excitation signal 5 - 60 mV p-p (0.1 mV resolution).
     - automated frequency sweeps and time sweeps.
     - automated data logging to an Excel or .csv file. 
     - lifetime software updates, distributed online.
     
Applications:

     - TEER measurement for up to four Endohm-6, Endohm-12 or Endohm-24SNAP chambers.

QPS1010:
     - quad pump with four small pump heads.
     - 4 x 4 x 4 in. (approx. 10 x 10 x 10 cm) footprint.
     - Peristaltic Mode: 5 uL/min. to 8 uL/min. flow rate.

     - Steady-State Mode: 5 uL/min. to 8 uL/min. at 0-150 bpm.
     
Applications:

     - any protocol in which steady-state or pulsatile fluid/media flow is required.
     - testing the hemodynamic properties of blood vessels in varying conditions.
     - replication of peristaltic action for the gastrointestinal tract.
     - replication of pulsatility for the cardiac cycle.

QPL1010:
     - quad pump with four large pump heads.
     - 6 x 6 x 5 in. (approx. 15 x 15 x 13 cm.) footprint.
     - Peristaltic Mode: 0.1 mL/min. to 20 mL/min. flow rate.

     - Steady-State Mode: 0.1 mL/min. to 12 mL/min. at 0-150 bpm.
     
Applications:

     - any protocol in which steady-state or pulsatile fluid/media flow is required.
     - testing the hemodynamic properties of blood vessels in varying conditions.
     - replication of peristaltic action for the gastrointestinal tract.
     - replication of pulsatility for the cardiac cycle.

QPP1010:
     - max flow rates:
       7 mL/min. (liquid)
       42 mL/min. (gas)
     - max pressures:
       600 mbar (liquid)
       100 mbar (gas)
     
Applications:

     - experiments requiring low rates of laminar fluid perfusion.
     - used in conjunction with single-fiber cell culture modules.

CAR1010:
     - 1.7 inch (4.3 cm) x 19 fiber

Applications:
     - growth of endothelial, epithelial, glial and pericyte cells. 
     - drug permeability and absorption through blood-brain barrier.
     - passage of nanocarriers, viral vectors, DNA, RNA, mRNA, siRNA, etc.
     - vascular dynamics / physiology of arterial and venous blood vessels.
     - effect of mechanical trauma on biological fluid compartments.

CAR1020:
     - 4.5 inch (11.5 cm) x 20 fiber

Applications:
     - growth of endothelial, epithelial, glial and pericyte cells. 
     - drug permeability and absorption through blood-brain barrier.
     - passage of nanocarriers, viral vectors, DNA, RNA, mRNA, siRNA, etc.
     - vascular dynamics / physiology of arterial and venous blood vessels.
     - effect of mechanical trauma on biological fluid compartments.

CAR1030:
     - 1.7 inch (4.3 cm) x 19 fiber
     - transmural openings >10 um


Applications:
     - growth of endothelial, epithelial, glial and pericyte cells. 
     - drug permeability and absorption through blood-brain barrier.
     - passage of nanocarriers, viral vectors, DNA, RNA, mRNA, siRNA, etc.
     - extravasation of leukocytes, stem cells, extracellular vesicles, etc.
     - metastasis of tumor cells between biological compartments.

CAR1040:
   
 - 1.7 inch (4.3 cm) x 19 fiber
     - 5 mm or 8 mm viewing window


Applications:
     - growth of endothelial, epithelial, glial and pericyte cells. 
     - drug permeability and absorption through blood-brain barrier.
     - passage of nanocarriers, viral vectors, DNA, RNA, mRNA, siRNA, etc.
     - microscopic slide mount added for confocal microscopy.

CAR1050:
     - 1.7 inch (4.3 cm) x 19 fiber
     - 5 mm or 8 mm viewing window 
     - transmural openings >10 um


Applications:
     - growth of endothelial, epithelial, glial and pericyte cells. 
     - drug permeability and absorption through blood-brain barrier.
     - passage of nanocarriers, viral vectors, DNA, RNA, mRNA, siRNA, etc.
     - extravasation of leukocytes, stem cells, extracellular vesicles, etc.
     - metastasis of tumor cells between biological compartments.
     - microscopic slide mount added for confocal microscopy.

CAR1060:
     - 1.7 inch (4.3 cm) x 1 fiber
     - piezo micro-pump only


Applications:
     - growth of endothelial, epithelial, glial and pericyte cells. 
     - drug permeability and absorption through blood-brain barrier.
     - passage of nanocarriers, viral vectors, DNA, RNA, mRNA, siRNA, etc.
     - extravasation of leukocytes, stem cells, extracellular vesicles, etc.
     - metastasis of tumor cells between biological compartments.
     - flat fiber enclosure for on-stage microscopy.

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